Part:BBa_K3893026

luxI constitutive expression

Figure 1. SBOL Visual representation of the part

Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893027, is a component of the intermediate part K3893029 to assemble the release system device (K3893030) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

• BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
• BBa_K2656009: the B0030 strong ribosome biding site in its Golden Braid compatible version
• BBa_K2656019: Codon-optimized foe E. coli, luxI sequence in its Golden Braid standardized version
• Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656019 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence C0161adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon-optimized for E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 11
  Illegal NheI site found at 34
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]